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Phusion taq polymerase manual

 
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MessagePosté le: Mar 2 Jan - 00:49 (2018)    Sujet du message: Phusion taq polymerase manual Répondre en citant

Download >> Download Phusion taq polymerase manual

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24 Dec 2017 Taq polymerase can be chemically inactivated at low temperatures, and this modification can be reversed at a high temperature; this temperature-dependent reversible modification of the Taq protein, AmpliTaq Gold, can be used as the hot start PCR enzyme [2]. Phusion DNA polymerase fuses Pfu DNA
PCR Protocol for Phusion® High-Fidelity DNA Polymerase (M0530). Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors,
Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors,
DNA Polymerase. Phusion DNA Polymerase (2U/µl) is provided with 5x Phusion. HF Buffer and 5x Phusion GC Buffer. Both buffers contain. 1.5 mM MgCl2 at final reaction concentrations. Separate tubes of DMSO and 50 mM MgCl2 solutions are provided for further optimization. 3.1 Basic reaction conditions for PCR
23 Sep 2016 Due to the nature of Phusion DNA. Polymerase, the optimal reaction conditions may differ from. PCR protocols for standard DNA polymerases. Due to the high salt concentration in the reaction buffer, Phusion DNA. Polymerase tends to work better at elevated denaturation and annealing temperatures.
Phusion DNA Polymerases have become the first choice in high-performance PCR for demanding applications including massively-parallel, high-throughput sequencing of whole genomes. All standalone enzymes are supplied with HF buffer for highest fidelity and GC buffer for amplification of difficult/GC rich targets.
29 Jan 2015 This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530)
Instruction Manual. Phusion® High-Fidelity PCR Kit. POLYMERASES & AMPLIFICATION. NEB #E0553S/L. 50/200 reactions. Version 1.4 2/15 be INSPIRED drive DISCOVERY stay GENUINE
DNA Polymerase gene. Applications: • PCR. • Cloning. • Long or Difficult Amplification. • High-throughput PCR. Supplied in: 20 mM Tris-HCl (pH 7.4 @ 25°C),. 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50% glycerol. Reagents Supplied with Enzyme: 5X Phusion HF Buffer. 5X Phusion GC Buffer.

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